Detergent (sodium dodecyl sulfate) shock proteins in Escherichia coli

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Detergent (sodium dodecyl sulfate) shock proteins in Escherichia coli.

The protein composition of Escherichia coli W3110 grown in the presence and absence of 5% sodium dodecyl sulfate (SDS) was examined by two-dimensional gel electrophoresis. In SDS-grown cells, at least 4 proteins were turned on, 13 were turned off, 15 were elevated, and 15 were depressed. The 19 unique and elevated SDS-induced spots constituted 7.91% of the total 35S-labeled protein. There was n...

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Sulfate) Shock Proteins in Escherichia coli

The protein composition of Escherichia coli W3110 grown in the presence and absence of 5% sodium dodecyl sulfate (SDS) was examined by two-dimensional gel electrophoresis. In SDS-grown cells, at least 4 proteins were turned on, 13 were turned off, 15 were elevated, and 15 were depressed. The 19 unique and elevated SDS-induced spots constituted 7.91% of the total 35S-labeled protein. There was n...

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Growth of Sodium Dodecyl Sulfate Micelles with Detergent Concentration

Sodium alkyl sulfate micelles are predicted to grow as a power law of the total counterion salt concentration, Yaq, in the aqueous phase as follows: NA = K~(Y~~)Y, where N A is the micelle aggregation number and ~2 and y are constants which are estimated from the critical micelle concentration and the apparent degree of counterion attachment. For sodium dodecyl sulfate, NA = 162(Y,q)1/4 is pred...

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Sodium dodecyl sulfate hypersensitivity of clpP and clpB mutants of Escherichia coli.

We studied the hypersensitivity of clpP and clpB mutants of Escherichia coli to sodium dodecyl sulfate (SDS). Both wild-type E. coli MC4100 and lon mutants grew in the presence of 10% SDS, whereas isogenic clpP and clpB single mutants could not grow above 0.5% SDS and clpA and clpX single mutants could not grow above 5.0% SDS. For wild-type E. coli, cellular ClpP levels as determined by Western...

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Escherichia coli heat-shock proteins

lstituto di Microbiologia’ and lstituto di Clinica Dermosif ilopaticaz, Seconda Universita degli Studi di Napoli, Facolta di Medicina e Chirurgia, Larghetto S.Aniello a Caponapoli, 2,801 38 Napoli, Italy Bacterial heat-shock proteins (HSPs) from Escherichia co/i (GroES, GroEL and DnaK) were studied for their ability to induce by themselves the expression and release of tumour necrosis factor-a ...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1991

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.173.1.229-233.1991